Purification, Characterization and Antitumor Activity of L-asparaginase from Penicillium brevicompactum NRC 829
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چکیده
منابع مشابه
Purification and some kinetic parameters of NAD glycohydrolase from Penicillium brevicompactum NRC 829
The current work reports the purification and characterization of some kinetic properties of nicotinamide adenine dinucleotide glycohydrolase (NADase) from Penicillium brevicompactum NRC 829. The enzyme was purified about 286 fold with a specific activity of 400 U/mg protein through a 3-step purification procedure. The purified NADase was shown to have a relative low molecular mass of 48 kDa by...
متن کاملPurification and characterization of the enzymes involved in nicotinamide adenine dinucleotide degradation by Penicillium brevicompactum NRC 829
The present study was conducted to investigate a new pathway for the degradation of nicotinamide adenine dinucleotide (NAD) by Penicillium brevicompactum NRC 829 extracts. Enzymes involved in the hydrolysis of NAD, i.e. alkaline phosphatase, aminohydrolase and glycohydrolase were determined. Alkaline phosphatase was found to catalyse the sequential hydrolysis of two phosphate moieties of NAD mo...
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L-asparaginase is an anti-neoplastic agent used in the lymphoblastic leukaemia chemotherapy. In the present study a novel strain, Streptomyces gulbargensis was explored for the production of extra-cellular L-asparaginase using groundnut cake extract. The optimum pH, temperature, inoculum size and agitation speed for enzyme production were pH 8.5, 40°C, 1x10(8)spores/ml and 200 rev/min respectiv...
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Homogeneols L-asparaginase with anti-lymphoma activity was prepared from Vibrio succinogenes, an anaerobic bacterium from the bovine rumen. An overall yield of pure L-asparaginase of 40 to 45% and a specific activity of 200 +/- 2 IU per mg of protein was obtained. The pure enzyme can be stored at -20 degrees for at least 3 months with no loss of activity. The isoelectric point of the L-asparagi...
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L-asparaginase is an enzyme that catalyzes the conversion of L-asparagine to L-aspartate and ammonia. The important application of the L-asparaginase enzyme in using it as chemotherapeutic for its anticarcinogenic potential .In the present study a novel strain, AcinetobacterbaumanniiR7 was explored for the production of extracellular Lasparaginase .This enzyme was purified by single chromatogra...
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ژورنال
عنوان ژورنال: British Microbiology Research Journal
سال: 2012
ISSN: 2231-0886
DOI: 10.9734/bmrj/2012/1735